How Combined Medical Technologies Create A Viable Bone Matrix

12 May 2020

The regeneration of bone that is capable of growth during the point of care in a hospital room is critical for a successful bone graft surgery. Approximately 83% of bone grafting uses autogenous grafts and 17% involve artificial substitutes. The medical technologies for the three elements of in-situ tissue engineering has proven to be predictably effective and safe. Here’s how medical technologies are combined to create a viable bone matrix for predictable bone regeneration.

viable bone matrix

Cells

Mesenchymal Stem Cells are first obtained using one of two technologies. The first technology is known as the Harvest Technologies System. It aspirates around 60 ml of autologous bone marrow from the posterior or anterior ilium. The aspirate is added to a transfer bag that contains four ml of ACDA anticoagulant. Then 60 ml of this mixture is transferred from the bag into a canister containing a floating shelf that separates osteoprogenitor cells from the bone marrow plasma.

The second technology is known as the Lenkbaar Marrow Marxman. It also aspirates bone marrow but has the capability of penetrating cortical surfaces where more mesenchymal stem cells reside. Only 10 ml of this material is needed.

Signal

Recombinant human Bone Morphogenetic Protein-2 (BMP-2) is known to be the signal for bone regeneration. It’s a protein that’s coded from chromosome 20 and separated from it by using restricted fragment enzymes. These enzymes are then attached to a plasmid, which is a bacterial extranuclear DNA ring. The injected plasmid is introduced into a Chinese Hamster Ovarian cell that produces a large amount of BMP-2 as well as hamster proteins.

Nanofiltration and electrophoresis are used to separate the human BMP-2, which is then freeze-dried for clinical use into a white crystallized powder. Before use, a dose of the freeze-dried crystals is placed in a pre-set package of sterile water. It’s then placed onto an acellular bovine collagen sponge for 15 minutes.

Matrix

The viable bone matrix is derived from the stem cell plasma component which contains various cell adhesion molecules that will act as a scaffold where the unmineralized organic component of bone can be deposited. Freeze-dried cancellous allogeneic bone is also used to build the matrix for the in-situ bone tissue engineering.

Application

During the bone repair surgery, the matrix is clotted with a mixture of calcium chloride into either recombinant human thrombin or lyophilized bovine thrombin. This clotting causes cell adhesion molecules to bind to the allogeneic bone surface and release various growth factors. A sponge that is wetted with this mixture is cut up by the surgeon into small squares. This is done to help create a space so that functional bone can fully grow, remodel, and fuse to be able to host bone for dental implants in the jaw.

In-situ bone tissue engineering is predictable for creating a viable bone matrix due to its sound wound healing and embryologic principles. The simplicity and safety are beneficial for both the surgeon and the healthcare system as a whole. Not only does it reduce operating and hospitalization time for graft delivery, but it also reduces the amount of pain and post-operative care for the patient.

Selecting the Right Bone Graft Delivery System

12 May 2020

When it comes time to select a bone graft delivery device or universal graft gun, things can get even more complicated. That’s why we’ve put together this list including a few simple tips to keep in mind when you’re trying to select the right bone graft delivery system.

Examine Current Equipment

Were you aware of the fact that the U.S. medical device market accounts for 40% of the global medical device market? The U.S. exports $44 billion in medical devices each year, according to the Department of Commerce. As a result, it’s safe to assume that you probably have some level of experience with universal graft guns, or some other type of bone graft delivery system. Even if you are not entirely satisfied with that piece of equipment, there are valuable lessons you can learn from it. As a matter of fact, your current equipment and set up may be the single most valuable asset you have to help you determine what you are (and aren’t) looking for in a new bone graft delivery system. Be sure to take this into account as you begin your search for the right equipment going forward.

Ask for Team Input

Your staff members, especially those who have extensive experience with universal graft guns, are particularly important to consult when you’re making a decision. Be sure to ask relevant staff members and surgeons what types of features they would find useful in a graft gun. Conversely, don’t forget to ask for their input on the previous shortcomings of older, outdated equipment. This type of experiential feedback can prove to be invaluable as you continue your search for the right bone graft delivery system. After all, it’s hard to find a better opinion than those of the employees who have extensive experience using the devices you’re looking for.

Ensure Excellent Quality

You should do your best to put ample time and energy into finding the right supplier for your equipment. Get a sense of the quality and reputation of the company you’re considering prior to making any sort of commitment. Chances are that if a particular bone graft delivery system has a strong reputation in the industry, then their products are ones that you know you can trust at your medical facility. Of course, the opposite holds true as well. Reading in-depth reviews by bone graft specialists can really help highlight some of the most important considerations you should be keeping in mind when looking at any prospective bone graft technology supplier.

At SurGenTec, we offer a wide range of supplies for healthcare professionals. If you’re looking for products that offer state of the art bone grafting, then look no further. Whether you’re in need of a universal graft gun, devices to streamline your bone graft surgery process, or any other type of bone grafting technology, then you can rest assured that our products deliver.